Abstract
This project contains physiological and proteomic measurements from a
controlled laboratory experiment designed to investigate how food
availability, thermal history, and sirtuin activity influence the response
of the California mussel (Mytilus californianus) to aerial heat stress.
Mussels were acclimated for three weeks to four combinations of food
ration (low or high) and daily emersion temperature (20 or 30 °C) in
tidal-simulation tanks that reproduced natural circadian and tidal cycles.
Following acclimation, mussels were exposed to an acute aerial heat stress
(33 °C), either with or without prior exposure to the sirtuin inhibitors
nicotinamide and suramin, and allowed to recover under their respective
acclimation conditions. The dataset includes label-free proteomic analyses
of gill tissue, measurements of ciliary activity and particle transport
velocity, clearance rates, valve gape and siphon activity, and associated
experimental metadata. Together, these data span multiple levels of
biological organization, linking molecular responses with whole-organism
feeding physiology during recovery from heat stress. The dataset provides
a resource for investigating the interactive effects of food availability,
thermal acclimation, and sirtuin-dependent regulation on physiological
performance and cellular stress responses in marine bivalves.