Identification of okadaic acid production in the marine dinoflagellate Prorocentrum rhathymum from Florida Bay

Tianying An; Jamie Winshell; Gloria Scorzetti; Jack W. Fell; Kathleen S. Rein

doi:10.1016/j.toxicon.2009.08.018

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Identification of okadaic acid production in the marine dinoflagellate Prorocentrum rhathymum from Florida Bay

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Identification of okadaic acid production in the marine dinoflagellate Prorocentrum rhathymum from Florida Bay

Tianying An, Jamie Winshell, Gloria Scorzetti, Jack W. Fell and Kathleen S. Rein

Toxicon (Oxford), Vol.55(2), pp.653-657

02-01-2010

DOI: https://doi.org/10.1016/j.toxicon.2009.08.018

PMCID: PMC2813983

PMID: 19735671

Abstract

ADAM derivative

Diarrheic shellfish poisoning (DSP) toxins

HPLC–FLD

LC-MS

Okadaic acid

Prorocentrum rhathymum

Extracts of fifty-seven newly isolated strains of dinoflagellates and raphidophytes were screened for protein phosphatase (PP2A) inhibition. Five strains, identified by rDNA sequence analysis as Prorocentrum rhathymum, tested positive and the presence of okadaic acid was confirmed in one strain by HPLC-MS/MS and by HPLC with fluorescence detection and HPLC-MS of the okadaic acid ADAM derivative. Quantitation of the ADAM derivative indicated that the concentration of okadaic acid in the culture medium is 0.153 μg/L.

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Title: Identification of okadaic acid production in the marine dinoflagellate Prorocentrum rhathymum from Florida Bay
Creators: Tianying An - Florida International University
Jamie Winshell - Florida International University
Gloria Scorzetti - University of Miami
Jack W. Fell - University of Miami
Kathleen S. Rein - Florida International University
Publication Details: Toxicon (Oxford), Vol.55(2), pp.653-657
Publisher: Elsevier Ltd; OXFORD
Number of pages: 5
Grant note: National Science Foundation: 0432368 National Institute of Environmental Health Sciences (NIEHS): P50 ES12736-01, S11 ES11181 Division Of Ocean Sciences; Directorate For Geosciences: 0432368
This work was supported by the NSF-NIEHS Oceans and Human Health Center Program (National Science Foundation grant 0432368 and NIEHS grant P50 ES12736-01). Grant support was also provided by the National Institute of Environmental Health Sciences (NIEHS) Grant S11 ES11181.
Identifiers: 99383964032806570
Academic Unit: Department of Marine & Earth Sciences
Language: English
Resource Type: Journal article

Identification of okadaic acid production in the marine dinoflagellate Prorocentrum rhathymum from Florida Bay

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