Abstract
Optical fiber-based biosensors have proven to be a powerful platform for chemical and biological analysis due to their compact size, fast response, high sensitivity, and immunity to electromagnetic interference. Among the various fiber designs, tapered optical fibers have gained prominence due to the increased evanescent fields that significantly improve light–analyte interactions, making them well-suited for advanced sensing applications. At the same time, advances in microfluidics have allowed for the precise control of small-volume fluids, supporting integration with optical fiber sensors to create compact and multifunctional optofluidic systems. This review explores recent developments in optical fiber optofluidic sensing, with a focus on two primary architectures: in-fiber and outside-fiber platforms. The advantages, limitations, and fabrication strategies for each are discussed, along with their compatibility with various sensing mechanisms. Special emphasis is placed on tapered optical fibers, focusing on design strategies, fabrication, and integration with microfluidics. While in-fiber systems offer compactness and extended interaction lengths, outside-fiber platforms offer greater mechanical stability, modularity, and ease of functionalization. The review highlights the growing interest in tapered fiber-based optofluidic biosensors and their potential to serve as the foundation for autonomous lab-on-a-fiber technologies. Future pathways for achieving self-contained, multiplexed, and reconfigurable sensing platforms are also discussed.